Figure 1.

PB-MNC Reprogramming by SeV

PB-MNCs from healthy donors and PKD patients were reprogrammed by SeV expressing OCT4, SOX2, KLF4, and cMYC mRNAs. Several lines from a healthy donor (PB2iPSC), patient PKD2 (PKD2iPSC), and patient PKD3 (PKD3iPSC) were isolated, expanded, and characterized.

(A) Diagram of the reprogramming protocol.

(B) Representative microphotographs of different iPSC lines derived from PB2 MNC, PKD2 MNC, or PKD3 MNC. Scale bars represent 200 μm.

(C) Sanger sequencing of each patient-specific mutation in the PKLR gene in PB2iPSC, PKD2iPSC, and PKD3iPSC. ^∗Mutations present in patient PKD2. ^#Mutation present in patient PKD3.

See also Figures S1 and S2.