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. 2015 Oct 29;5(6):1210–1225. doi: 10.1016/j.stemcr.2015.09.020

Figure 2.

Figure 2

Phenotypic Characterization of Tonsillar Epithelial Cells According to Their Surface Expression of CD44 and NGFR

(A) Experimental protocol for analyzing single CD45− (non-hematopoietic) and CD31− (non-endothelial) cells according to their expression of CD44 and NGFR.

(B) FACS gating strategies. SSC, side scatter; FSC, forward scatter; FSC-W, FSC width.

(C) IHC analysis of CD45−CD31−cells isolated by FACS at purities of >90% according to their expression of CD44 and NGFR and cytospun prior to fixation and staining with antibodies to CK19, CK5, p63, and CK4. Inset, negative control. Scale bar, 20 μm.

(D) FACS plots for CD45−CD31− surface cells.

(E) FACS plots for CD45−CD31− crypt cells.

(F) Percentages of cells positive for the indicated intracellular proteins. Values shown are the mean ± SD. At least three independent samples were evaluated for each marker.