Table 1. Enhancement of Lactoferrin uptake in cells by GAPDH.

Cell type	Fold change in Lactoferrin MFI of cells internalizing both GAPDH and Lf vs. only lactoferrin +ve cells
Mouse Enterocytes	5.52*
Mouse Hepatocytes	4.28*
Mouse Bone Marrow cells	3.7*
Mouse Spleen Macrophages	2.1*
Mouse Peritoneal Macrophages (Ex vivo)	1.17*
Mouse peritoneum cells (In-Vivo)	6.1*
Human Lymphocytes	1.27*
J774	2.62*
PMA activated THP1	2.07*
Non activated THP1	No significant uptake of Lf
CHO	4.95*
CHO-TRVb	2.83*
K562	1.6*
Neuro-2A (N2A)	4.02*

Relative trafficking of Lf by sGAPDH as compared to surface receptors. 5 × 10⁵ cells were incubated at 37 °C for 30 minutes with either; (i.) only lactoferrin Alexa-633 (5 μg), (ii.) only GAPDH-FITC (10 μg) or (iii.) both (i.) & (ii.). After internalization cells were washed, pronase treated and analyzed by FACS. A two-color quadrant analysis was done to see relative trafficking as described in methods, *p < 0.001 compared to Lf internalized via surface receptors only. n = 10⁴ cells in each case.