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. 2015 Dec 17;5:17936. doi: 10.1038/srep17936

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(a) A size exclusion chromatogram. KMTR2 (upper) or the LkN53R mutant (lower) was mixed with the ecTRAIL-R2:Fc fusion protein at a molar ratio of 1:1 and loaded onto Superdex 200 10/300 GL gel filtration column. Monomer antibodies and ecTRAIL-R2:Fc were also loaded independently. (b) Cell surface TRAIL-R2 immune complexes on Colo205 cells visualized by confocal microscopy (×40). The cells were incubated with monomer KMTR2 (upper) and the LkN53R mutant (lower) at 37°C for 1.5 h and then labeled with Alexa488.