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. 2015 Dec 15;6:10166. doi: 10.1038/ncomms10166

Figure 10. Ceramide compromises ‘browning' function of beige adipocyte.

Figure 10

(a,b) mRNA levels of thermogenic related genes in beige adipocytes after 8 h of indicated doses of ceramide treatment (a) or after the indicated time point of 25 μM ceramide treatment (b). Six to seven replicates of three separate experiments. Data are presented as mean±s.d. Two-tailed Student's t-test. *P<0.05, **P<0.01 compared with control. (c) Oxygen consumption rate (OCR) of beige adipocytes after 8 h of indicated concentration ceramide treatment in the presence of 1 μM oligomycin (ATP synthase inhibitor), 1 μM FCCP (uncoupling agent) and 1 μM rotenone (complex I inhibitor) and 1 μM antimycin A (complex III inhibitor). Six replicates of three separate experiments. (d) Basal and maximal respiration of beige adipocytes after 8 h of indicated concentration ceramide treatment. Six replicates of three separate experiments. Data are presented as mean±s.d. Two-tailed Student's t-test. **P<0.01 compared with control. (e) Cell viability after 24 h of the indicated doses of ceramide treatment. (a–e) Six replicates of three separate experiments. Data are presented as mean±s.d. Two-tailed Student's t-test.