Skip to main content
. 2015 Dec 14;6:10119. doi: 10.1038/ncomms10119

Figure 8. Reducing RCAN1 gene dosage improves neuronal survival and target innervation in Dp(16)1Yey/+ mice.

Figure 8

(a–d) Genetic reduction of RCAN1 ameliorates the loss of sympathetic neurons in Dp(16)1Yey/+ mice. P0.5 SCGs from Dp(16)1Yey/+:RCAN+/− mice show increased size and neuronal numbers compared with Dp(16)1Yey/+ litter-mates. SCGs were visualized by TH immunohistochemistry and cell counts were performed on Nissl-stained tissue sections. Results are the mean±s.e.m. from n=5 mice per genotype. *P<0.05, ***P<0.001. (e–l) TH immunohistochemistry reveals a marked improvement of sympathetic innervation in target tissues in P0.5 Dp(16)1Yey/+:RCAN+/− mice compared with Dp(16)1Yey/+ litter-mates. Innervation of nasal epithelium is shown in e–h and that of salivary glands in i–l. Quantification of innervation density (h, nasal epithelium; l, salivary glands) was estimated from n=6 mice per genotype. Values are the mean±s.e.m. The results are represented as a percentage of the mean for wild-type mice. *P<0.05, **P<0.01, ***P<0.001. Scale bar, 100 μm for a,b,c,i,j,k and 50 μm for e,f,g. Statistical analyses by one-way ANOVA and Tukey's post-hoc test for d,h,l.