Figure 2. Comparison of phenotypes and genotypes between parental ey^Δ877/Δ1 strain and HR revertants.

(a) Lateral pictures of wild type (left), parental ey^Δ877/Δ1 strain (centre), and HR revertant (right). The ey^Δ877/Δ1 strain shows the deformed, non-spherical compound eye (ce) phenotype. The HR revertant shows the normal eye phenotype and wild type strain. Scale bar indicates 100 μm. (b) Schematic illustration of genomic PCR to check the integration of attP into the Dma-ey Δ1 allele. Black thick bar indicates the Dma-ey Δ1 allele. Primers are shown by arrows. The attP knock-in results in 240 bp long PCR products, whereas no modification gives 173 bp long PCR products. (c) Agarose gel electrophoresis of genomic PCR products using HR revertant strains. 1 and 2 are derived from co-injection of TALEN mRNAs and targeting plasmid, whereas 3 is derived from co-injection of TALEN mRNAs and ssODN with 80 nt homology. attP knocked-in HR revertants showed 240 bp PCR products, whereas parental ey^Δ877/Δ1 strain showed 173 bp PCR products. M indicates the marker DNA.