Figure 1. Chloroamidine and elastase inhibitor decreased netosis induced by Leishmania amazonensis (La).

Neutrophils (NØ; 2 × 10⁶) were incubated with (A) Elastase inhibitor (E.i, 5 and 10 μM); (B) Chloroamidine (Cl-A, 12 μM) and (C) Myeloperoxidase inhibitor (MPOi, 300 nM), for 30 min and then, stimulated or not with PMA (100 nM) or promastigotes of L. amazonensis (1NØ: 0.1 La ratio) for 1 h. Following stimulation, DNA quantification was performed using PicoGreen assay kit in culture supernatants. Data normalized regarding spontaneous release of DNA representing the mean ± SEM from 17 (A), 7 (B,C) donors. *p < 0.0001 and **p < 0.05.