Figure 3. ROS and RNS role in NET release induced by Leishmania amazonensis (La).

Neutrophils (NØ; 2 × 10⁶) were incubated with (A) DPI (32 μM), (B) L-NAME (1 mM), (C,D) MitoTempo (100 μM) and (E) Allopurinol (1, 2 and 4 mM) for 30 min and then stimulated or not with promastigotes (1 NØ: 0.1La ratio) or PMA (100 nM) for 1 h, followed by DNA quantification using PicoGreen assay kit in culture supernatants. Data were normalized according spontaneous release of NET-DNA representing the mean ± SEM from 22 (A), 12 (B), 7 (C), 11 (D), 10 (E) and 11 (F) donors. *p < 0.0001 and **p < 0.05.