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. 2015 Dec 17;5:18295. doi: 10.1038/srep18295

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(A) Survival (%) from egg to eclosion of flies of the indicated genotypes, all bearing the da-GAL4 driver and the CG-9603 knockdown (RNAi) construct. Lines tested contained either no additional transgene (–), vector only (v), wild-type (wt) or mutated AOX (mut), in each case on chromosome 3. (B) Boxplot of climbing index of flies of the indicated genotypes. All flies carried the elav^C155-GAL4 driver on chromosome X plus UAS-Dcr2 with or without the CG9603 knockdown (RNAi) construct on chromosome 2, and the indicated AOX transgene on chromosome 3 (AOX^7.1 is the ΦC31-targeted insertion). Bars indicate medians, boxes show the first and third quartiles percentiles, whiskers are plotted according to the Tukey scheme (Krzywinski and Altman, 2014). Significant differences based on ANOVA are indicated by horizontal lines (black, red) denoting p < 0.05 and 0.001, respectively. A single outlier point is indicated by an open circle.