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. 2015 Dec 17;5:18286. doi: 10.1038/srep18286

Figure 2. Phorbol ester and ionomycin treatment trigger increased APP expression.

Figure 2

Treatment of QBI293 cells stably-expressing hAPP with PMA (1 μM) for 36 h resulted in increased (A) APP mRNA [R.Q. values of APP/GAPDH are relative to vehicle-treated cells], (B) APP protein expression [(top) representative blot and (bottom) quantification of APP detected with 5685 antibody, with values relative to vehicle-treated cells and normalization to α-tubulin], and (C) Aβ1-40 production [values are relative to vehicle-treated cells and normalized to total cellular protein content]. Addition of ionomycin (1 μM) to PMA further increased APP mRNA and protein expression, and Go6983 treatment inhibited these increases. Statistical analyses consisted of a mixed-effects model, with values representing estimates from the least squares means fit of the mixed procedure from 2–4 independent studies with 2–5 replicates for each treatment/study. Error bars represent SEM; *p < 0.05; **p < 0.01; ***p < 0.001.