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. 2015 Dec 17;5:18286. doi: 10.1038/srep18286

Figure 4. Activation of eicosanoid receptors increases APP expression in primary rat hippocampal neurons.

Figure 4

Rat hippocampal neurons that were grown in culture for 14 days were treated for 2 days with receptor agonist alone or in combination with a specific receptor antagonist, and APP expression was compared to that of control cultures by immunoblotting (22C11 antibody). (A) IBOP (100 nM) increased APP expression and was blocked by the TP receptor antagonist, CNDR-51280 (10 μM); (B) LTD4 (1 μM) increased APP expression and was inhibited by the CysLT1 receptor antagonist, Pranlukast (1 μM); and (C,D) 17PGE2 (1 μM) enhanced APP expression, and was not inhibited by the EP1 receptor antagonist, ONO8711 (20 nM), but was blocked by the EP3 receptor antagonist, L798,106 (30 nM). Statistical analyses consisted of a mixed-effects model, with values representing estimates from the least squares means fit of the mixed procedure from 3 independent studies, with 4 replicates per study. Error bars represent SEM; **p < 0.01; ***p < 0.001.