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. 2015 Dec 17;5:18286. doi: 10.1038/srep18286

Figure 5. Activation of eicosanoid receptors in primary neurons with saturating and sub-saturating concentrations of receptor agonists.

Figure 5

Rat hippocampal neurons (A) or mouse cortical neurons (B) that were grown in culture for 14 days were treated for 2 days with vehicle or high concentrations of IBOP (100 nM), LTD4 (1 μM), 17PGE2 (1 μM), or the combination of these agonists (I + L + 17). In (C), rat hippocampal neurons were treated with vehicle or sub-saturating concentrations of IBOP (50 nM; IBOP(L)), 17PGE2 (0.5 μM; 17PGE2(L)), or the combination of these agonists (I(L) + 17(L)). In addition, neurons were also treated with a high concentration of IBOP (100 nM; IBOP(H)). APP was quantified from immunoblots (22C11 antibody), and statistical analyses consisted of a mixed-effects model, with values representing estimates from the least squares means fit of the mixed procedure from 3–4 independent studies, with 2–3 replicates per study. Error bars represent SEM; *p<0.05, **p < 0.01; ***p < 0.001.