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. 2015 Dec 17;5:18286. doi: 10.1038/srep18286

Figure 6. Aged 5XFAD transgenic mice show increased levels of PGE2 and TXB2, as well as APP.

Figure 6

Whole hemisphere brain homogenates from 5XFAD mice and non-transgenic (non-Tg) littermates that were 1.5 or 6.5 months of age were analyzed by LC-MS/MS for (A) PGE2 and (B) TXB2 (n = 3–4 per group). In (C), the level of total APP expression in cortical and hippocampal brain homogenates from 1.5 month old and 6.0 month old 5XFAD mice was determined by immunoblotting (22C11 antibody), with normalization to GAPDH. Lane loading in the immunoblot was randomized for study blinding during quantification (n = 5 per group; 2 females (F) and 3 males (M) in each age group). Statistical analyses consisted of a one-way ANOVA with a Tukey’s multiple comparison test for (A) and (B), and a two-tailed t-test in (C). Error bars represent SEM; *p < 0.05; **p < 0.01; ***p < 0.001.