Figure 4.

hESC-Derived Insulin-Positive Cells from Both Species Expressed Similar β Cell Transcription Factors Except MAFA Expression Was More Heterogenous in Mice.

(A) Representative immunofluorescent staining of engrafted cells for insulin (red), glucagon (blue), and various transcription factors (green): PDX1, NKX6.1, and NKX2.2 (scale bars, 100 μm).

(B) Three different regions of insulin-positive cells (red) from each graft illustrate the heterogeneity in nuclear MAFA expression (green; white arrows show examples of insulin-positive cells without nuclear MAFA immunoreactivity). Scale bars, 50 μm. DAPI nuclear staining is shown in gray.

See Figure S6 for additional immunofluorescent staining of mature β cell markers in hESC-derived grafts.