Figure 7.

Grafts from Rats Were Enriched for Genes Related to Extracellular Matrix Composition and Remodeling and Were Densely Integrated with Collagen Fibers

(A) List of all genes significantly enriched >5-fold in grafts from rats relative to mice at 22 weeks post-transplantation based on microarray analysis (n = 3 animals/group).

(B) Plasma concentration of human MMP1 and MMP3 at either 22 or 33 weeks post-transplantation (n = 4–6 animals/group). ^∗p < 0.05, ^∗∗p < 0.01; two-tailed t test. Data are presented as mean ± SEM plus individual biological replicates.

(C) Masson’s trichrome staining of adult human pancreas shows blue collagen fibers surrounding a duct (white arrow) and an islet (yellow arrow). Scale bar, 100 μm.

(D) Masson’s trichrome staining of hESC-derived grafts at 22 weeks post-transplantation. Low-magnification images of the whole grafts are shown on the left (scale bars, 500 μm). White dashed lines delineate graft and kidney tissue. White boxes highlight the two regions (R1 and R2) shown at higher magnification to the right (scale bars, 100 μm).

See also Figure S7.