Skip to main content
. 2015 Dec 15;6:8964. doi: 10.1038/ncomms9964

Figure 5. 9+2 axoneme shortening and restructuring to 9v is associated with a reduction in IFT.

Figure 5

(a) Frames from Supplementary Movie 2 illustrating IFT52::eGFP localisation and movement in IFT particles in EPPs. The approximate position of the basal body (BB) and flagellar pocket (FP) are indicated. (b,c) IFT activity in EPPs, shown by (b) representative kymographs of IFT52::eGFP movement and (c) quantitation of fluorescence intensity moving with an anterograde (positive) or retrograde (negative) velocity. (d,e) IFT activity 4 h after transfer of EPPs to SM5.5, shown by (d) representative kymographs and (e) quantitation of fluorescence. Error bars indicate s.e. Horizontal scale bars represent 5 μm, vertical scale bars represent 5 s.