Figure 2.

Gene Expression during Differentiation

(A–F) Gene expression of the pluripotency-associated markers (A) OCT4 and (B) NANOG, the endoderm/hepatoblast markers (C) FOXA2 and (D) α fetoprotein (AFP), (E) hepatocyte nuclear factor-4-α (HNF4A), and (F) albumin (ALB) was analyzed on days 0, 3, 9, and 18, normalized to the housekeeping gene GAPDH, and expressed relative to hESCs. The results shown represent three biological replicates, and error bars represent SD. ^∗p < 0.05, ^∗∗p < 0.01; one-way ANOVA with Tukey post hoc test.