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. 2015 Nov 25;5(6):1250–1262. doi: 10.1016/j.stemcr.2015.10.016

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Endoderm and Hepatic Specification

(A and B) FOXA2 (A) and SOX17 (B) stained cells on MG, laminin 521, and laminin 111 on day 3 of differentiation. Cells were counterstained with Hoechst 33342. The percentage of positive cells and SD are shown. Images were taken at ×20 magnification.

(C–E) AFP (C), HNF4a (D), and CK19 (E) on MG, laminin 521, and laminin 111 on day 9 of differentiation. Cells were counterstained with Hoechst 33342. Data are presented as the mean of three independent experiments ± SD.

All images were taken at ×10 magnification.