Endoderm and Hepatic Specification
(A and B) FOXA2 (A) and SOX17 (B) stained cells on MG, laminin 521, and laminin 111 on day 3 of differentiation. Cells were counterstained with Hoechst 33342. The percentage of positive cells and SD are shown. Images were taken at ×20 magnification.
(C–E) AFP (C), HNF4a (D), and CK19 (E) on MG, laminin 521, and laminin 111 on day 9 of differentiation. Cells were counterstained with Hoechst 33342. Data are presented as the mean of three independent experiments ± SD.
All images were taken at ×10 magnification.