Figure 5.

Metabolic Function and Expression

(A and B) Expression of CYP2D6 (A) and CYP3A4 (B) on MG, LN521, and LN111 at day 24 of differentiation. Cells were counterstained with Hoechst 33342. Data are presented as the mean of three independent experiments ± SD. All images were taken at ×10 magnification.

(C) Cytochrome P450 metabolic activity of CYP1A2 and CYP3A of cells cultured on Matrigel (black columns), laminin 521 (shaded columns), and the laminin 111 mix (white columns). Data are presented as mean of six independent experiments. Error bars represent SEM.^∗p < 0.05, ^∗∗p < 0.01; one-way ANOVA with Bonferroni post hoc analysis. The dotted and continuous lines indicates the average CYP activity of primary hepatocytes in culture. PHH(F), primary human hepatocytes, female; PHH(M), primary human hepatocytes, male.