Table 3.
RLU/CO and Ct values for concordant and discordant digene HC2 and Roche COBAS results
| RLU/CO | Ct (all) | Ct (HPV 16 only) | Ct (HPV 18 only) | Ct (OHR only) | |
|---|---|---|---|---|---|
| Median (Q1, Q3) | Median (Q1, Q3) | Median (Q1, Q3) | Median (Q1, Q3) | Median (Q1, Q3) | |
| HC2+/COBAS+ | 54.9 (8.6, 368.4) | 30.2 (26.6, 34.2) | 29.4 (26.6, 34.2) | 30.7 (27.2, 35.0) | 30.3 (26.3, 33.8) |
| HC2+/COBAS− | 5.9 (2.7, 16.4) | – | – | – | – |
| HC2−/COBAS+ | 0.3 (0.2, 0.5) | 38.3 (36.8, 39.1) | 39.0 (38.0, 40.0) | 38.1 (37.0, 38.8) | 38.2 (36.7, 38.9) |
| HC2−/COBAS− | 0.1 (0.1, 0.2) | – | – | – | – |
Abbreviations: HC2 Digene Hybrid Capture® 2 high-risk HPV DNA test®, COBAS Roche cobas® 4800 HPV test, RLU/CO relative light unit/cutoff, Ct cycle threshold, Q1 first quartile, Q3 third quartile