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. 2015 Dec 16;15:964. doi: 10.1186/s12885-015-1980-8

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© Rivera et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — In vitro assay on phagocytic effect of activated macrophages against human tumor cells with or without mCD47 expression. Activated RAW 264.7 mouse macrophages were co-cultured with either PC3-mCD47 and PC3-GFP-Luc cell pair (a) or HCT116-mCD47 and HCT116-GFP-Luc cell pair (b). Tumor cell viability was determined by measuring intracellular luciferase activity and was presented as relative light unit (RLU). Cancer cells without macrophages co-culturing (the last column of each figure) were used as a positive control and macrophages without tumor cells were used as the negative control for the assay. Bars represent means plus standard deviations for two experiments, each with duplicate samples. ✱p < 0.05 as compared to the respective GFP-Luc control cells