Fig. 1.

Generation of stable α-gal epitope-expressing SKOV3 cell lines. a Following α1,3GT expressing lentivirus infection, GFP was observed on almost all the SKOV3-gal cells under fluorescent microscopy. No GFP expression was observed on the parental SKOV3 cells. Scale bars: 200 μm. b The expression of α1,3GT mRNA increased more than 250 times in SKOV3-gal cells compared with parental SKOV3 cells (**P < 0.01). c Only about 0.8 % of parental SKOV3 cells showed nonspecific fluorescence. d More than 99 % of SKOV3 transfectant expressed α-gal epitopes on the cell surface by flow cytometry