Maintained Cell Characteristics and Differentiation Capacity in Transduced mMABs
(A) No difference in expression of surface markers was observed between transduced mMABs and WT mMABs. All mMABs were positive for the typical markers (Sca-1, CD44, and CD140α) and negative for the endothelial marker CD31.
(B) Both transduced and WT mMABs were capable of fusing/differentiating toward myofibers, indicated via the presence of yellow fibers due to the overlay of GFP and red stained ACTA1. No GFP could be found in the myofiber differentiation of the C2C12s alone. The scale bar represents 100 μm.