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. 2015 Nov 28;5(6):1183–1195. doi: 10.1016/j.stemcr.2015.10.018

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

In Vitro Validation of Reporter Gene Expression in Differentiated Transduced mMABs

(A) Visualization of BLI signals in undifferentiated and differentiated mMABs indicated clear BLI signals in transduced mMABs in both conditions (n = 3 TR).

(B) Quantitative analysis of BLI signals indicated only a BLI signal in transduced mMABs. There was no difference that could be observed between BLI signals produced in undifferentiated and differentiated transduced mMABs after normalization to cell amount (mean ± SD).

(C) Quantitative analysis of tracer uptake demonstrated tracer uptake in both undifferentiated and differentiated Fluc-hNIS⁺ mMABs (n = 3 TR). There was no difference that was observed between both conditions in the Fluc-hNIS⁺ mMABs (mean ± SD).