Figure 6.

Monitoring of Intraarterial Injected Fluc⁺ and Fluc-hNIS⁺ mMABs via BLI and Small Animal PET

(A) 1 × 10⁶ Fluc⁺ and Fluc-hNIS⁺ mMABs were injected in the left and right femoral artery, respectively, of nude mice with acute muscle damage (n = 10 IR). After ¹²⁴I⁻ administration only in the right hind limbs, the Fluc-hNIS⁺ cells could be detected, while background signal was present in the left hind limbs (representative image of day 1 post-injection).

(B) Quantitative analysis resulted in significantly increased SUVs in the right hind limbs compared to the left hind limbs both on day 1 and day 3 (^∗∗∗p < 0.001) (mean ± SD).

(C) Representative BLI images indicate the feasibility to monitor both Fluc⁺ and Fluc-hNIS⁺ mMABs longitudinally via BLI.

(D) Quantitative analysis demonstrated a robust BLI signal, which remained stable starting from day 21 (mean ± SD) (see also Figure S1).