Figure 2.

Purification of Ventricular Cardiomyocytes from Differentiating mESCs through IRX4-2 MBs

(A) A schematic of the protocol to differentiate mESCs to the cardiac lineage. ESCs, mouse embryonic stem cells; EBs, embryoid bodies.

(B) Flow cytometric scattergrams showing the percentages of cells expressing both TNNT2 and MYL2 at differentiation day 18.

(C) A flow cytometry plot showing IRX4-2-MB-positive cells at differentiation day 18.

(D) Flow cytometric scattergrams showing the percentages of cells expressing both TNNT2 and MYL2 after FACS sorting with IRX4-2 MB.

All experiments were performed on three (B and D) or six (C) independent biological replicates.