Skip to main content
. 2015 Dec 8;5(6):1239–1249. doi: 10.1016/j.stemcr.2015.10.021

Figure 4.

Figure 4

Characterization of Purified Ventricular CMs through IRX4-2 MBs

(A) Immunocytochemistry for ACTN2, TNNT2, and MYH6/MYH7 on IRX4-2-MB-positive cells isolated from cardiomyogenically differentiated mESCs. Scale bars, 20 μm.

(B) Expression of GJA1 determined by immunocytochemistry on IRX4-2-MB-positive cells isolated from cardiomyogenically differentiated mESCs. Scale bars, 50 μm.

(C) mRNA expression of cardiac (Myh6/Myh7 and Tnnt2), ventricular (Myl2 and Irx4), atrial (Myl7), and non-cardiac genes (Acta2, Ddr2, and MyoD) in IRX4-2-MB-positive and -negative cells measured by qRT-PCR. y axis represents relative mRNA expression of target genes to GAPDH. ∗p < 0.05 compared to in IRX4-2-MB-negative cell group. Data are represented as mean ± SEM. All experiments were performed on three independent biological replicates.