Figure 1.

Differentiation of FOP hiPSCs into ECs and Pericytes

(A) Total SMAD1/5 and phospho-SMAD1/5 (pSMAD1/5) level in control hiPSCs (C3-3 and UE017C1) and FOP hiPSCs (F2-6 and F3-8). Note that the antibodies used here also may recognize SMAD8 and pSMAD8 bands.

(B) FACS analysis of EC marker (CD31) or pericyte marker (PDGFRβ) expression at differentiation days 10–12 is shown.

(C) Quantification of the FACS analysis data for relative percentage of CD31 and VE-cadherin double-positive ECs and PDGFRβ-positive pericytes. All values were adjusted to the control colony C3-3, which is defined as 1.

(D) Relative gene expression at different time points during the differentiation. ACTIN was used to normalize gene expression.

Data are presented as mean and SD from three independent experiments in (A), (C), and (D).