Figure 4.

Both SOX2⁺ Stem Cells and PIT1⁺ Cells Exhibit Reduced Proliferation in the Anterior Lobe of Rbp-J^f/f, Prop1-Cre Mutants at P1

(A–D) Double immunofluorescence labeling of SOX2/KI67 (A and B) and PIT1/KI67 (C and D) in wild-type control (A and C) and Rbp-J^f/f, Prop1-Cre mutants (B and D) at P1.

(E) Quantification of KI67⁺ proliferating cells in the anterior lobe in the control and mutant (CKO) at P1. Data are represented as mean ± SEM (n = 3 mice, ^∗p < 0.05).

(F) Quantification of SOX2⁺ and PIT1⁺ cells among KI67⁺ proliferating cells in the control and mutant. Data are represented as mean ± SEM (n = 3 mice, ^∗∗p < 0.01).

(G) Quantification of SOX2⁺ KI67⁺ cells among SOX2⁺ cells. Data are represented as mean ± SEM (n = 3 mice, ^∗p < 0.05).

(H) Quantification of PIT1⁺ KI67⁺ cells among PIT1⁺ cells. Data are represented as mean ± SEM (n = 3 mice, ^∗∗p < 0.01).

Scale bars, 100 μm.