Figure 1.

Negative Regulation of Nodule Formation by Gα Proteins.

(A) Nodulation phenotype of soybean Gα-RNAi transgenic hairy roots driven by FMV promoter using a construct targeting the switch region of Gα proteins.

(B) Nodulation phenotype of Gα-RNAi hairy roots driven by FMV promoter using a construct targeting the C-terminal region of Gα proteins.

(C) Nodulation phenotype of soybean hairy roots overexpressing individual Gα genes driven by CvMV or Enod40 promoter. Nodule numbers on transgenic hairy roots were counted at 32 dpi with B. japonicum and were compared with their respective EV-transformed control hairy roots. The data are average values from three biological replicates (40 to 50 individual plants/biological replicate containing transgenic nodulated roots). Asterisks indicate statistically significant differences compared with EV control (*P < 0.05; **P < 0.01; Mann-Whitney U test).