Figure 5.

MYB11 and ZML2 Bind Simultaneously to a AC-GAT(A/C) CRM That Is Present in the comt Promoter of Maize and Other Plant Species.

(A) Phylogenetic tree of COMT from several species. On the right, predictions of AC and GAT(A/C) elements and CRERs in the comt promoter of different species. AC elements are represented by lines in different shades of blue, and GAT(A/C) boxes are shown as purple lines. Predicted cis-regulatory modules are shaded in yellow.

(B) Sequential chromatin immunoprecipitation (ChIP-reChIP) using antibodies against MYB11 and ZML2. qPCR assays were performed for fragments A, B, C, C-II, C-III, and D of the comt promoter and for actin, copia, α-tubulin, and comt 3′UTR as a control. AC (gray boxes) and GATA (black boxes) elements in the maize comt promoter or 5′UTR are indicated at the bottom. Gray bars refer to ChIP-reChIP performed using MYB11 (ChIP) and ZML2 antibodies (reChIP). White bars refer to control ChIP-reChIP using ZML2 antibodies only. Results are represented as percentage of input. Error bars indicate the se obtained from three independent biological replicates. Statistical analysis was performed using Student’s t test (***P < 0.005).