Figure 7.

Schematic Representation of the DNA Binding Sites of MYB11, MYB31, MYB42, and ZML2 and Prediction of an AC-GAT(A/C) CRM in All the Lignin Genes Analyzed.

Blue, red, and green filled circles refer to MYB11, MYB31, and MYB42 binding sites identified by ChIP-qPCR assays, respectively. Predictions of AC and GAT(A/C) elements and CRERs in the promoter of different lignin genes were performed using the online Matrix-Scan software (see Methods) by scanning the upstream sequences with matrices representing binding motifs for the MYB transcription factors and ZML2. The AC elements are represented by blue (for MYB11), red (MYB31), and green (MYB42) lines, and GAT(A/C) boxes are shown as purple lines. Predicted cis-regulatory modules are shaded in yellow.