Figure 5.

Recruitment of the Exocyst Complex to the Appressorium Pore Is Septin-Dependent.

(A) Micrographs of exocyst subunit Sec6:GFP expressed in wild-type strain Guy11 and Δsep3, Δchm1, ΔnoxR, and Δmst12 mutants. Conidial suspensions at 5 × 10⁴ mL⁻¹ were inoculated onto glass cover slips and the expression of Sec6:GFP was checked at 4 and 24 h after inoculation. Bar = 10 µm.

(B) Bar chart showing percentage of appressoria expressing exocyst subunit Sec6:GFP at 4 h (black bars) and 24 h (gray bars) after inoculation. Values are mean ± sd for three repetitions of the experiment, n = 300.