Figure 8.

SMXL6, 7, and 8 Interact with TPR2.

(A) Interactions of TPR2 with SMXL6, 7, and 8 in yeast cells. Yeast cells were cotransformed with constructs encoding the binding domain (BD) fused to each SMXL and the activation domain (AD) fused to TPR2 and plated on selective media.

(B) Interactions in Arabidopsis protoplasts of SMXL6, SMXL7, or SMXL8 with TPR2. Protoplasts were prepared from wild type (Col-0) and cotransformed with genes encoding Flag-TPR2 and either GFP-SMXL or GFP-SMXLΔEAR (both for each of SMXL6, 7, and 8). After incubation for 12 h, cells were broken and immunoprecipitation (IP) with agarose-conjugated anti-GFP monoclonal antibody was performed, following which the Flag-TPR2 was detected with an anti-Flag monoclonal antibody, while GFP-SMXL and GFP-SMXLΔEAR proteins were detected with an anti-GFP monoclonal antibody.