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. 2015 Oct 16;27(10):2972–2990. doi: 10.1105/tpc.15.00399

Figure 4.

Figure 4.

Targeted UPLC-MS/MS Analysis of the Products Resulting from Yeast-Expressed CYP76C1 Activity on 8-Oxo-Linalool.

Samples were analyzed by UPLC-MS/MS using MRM. Left panels show a specific channel of MS/MS transition developed for the detection of 8-oxo-linalool (151.2 → 92.8 m/z). Right panels show a specific channel of MS/MS transition developed for the detection of 8-COOH-linalool (167.2 → 92.8 m/z).

(A) Mix of authentic standards.

(B) Methanol extract of the products from the conversion of 8-oxo-linalool by CYP76C1. Yeast microsomal membranes (final [P450] ∼100 nM) were incubated for 20 min with the substrate (200 µM) in the presence or absence (neg. control) of NADPH. Red panel shows the consumption of the substrate by CYP76C1 in presence of NADPH compared with the negative control. Minor amount of 8-COOH-linalool is detected in the negative control, probably due to 8-oxo-linalool autoxidation. 8-COOH-linalool was identified from its specific MS/MS and retention time compared with the authentic standard. More details and extended results are shown in Supplemental Figure 8. (4) 8-oxo-linalool and (9) 8-COOH-linalool.