Figure 3.

Aggregates Are Comprised of Cytoskeletal Proteins

(A) LRRK2 G2019S aggregates were positive (indicated by arrows) for βIII tubulin (green) and peripherin (red), tau (green), MAP2 (red), and LRRK2 (green). Aggregates were generally negative (indicated by arrowheads) for SNCA and ubiquitin (green).

(B–D) LRRK2 G2019S sensory neuron cultures display significantly increased levels of (B and C) tau and (B and D) phospho-tau by western blot and densitometry. ^∗p < 0.01 by ANOVA; n = 6 independent experiments.

The scale bar represents 50 μm. See also Figure S3.