Figure 4.

LRRK2 G2019S and LRRK2 G2019S het iPSC-Derived Sensory Neurons Display Abnormal Calcium Dynamics

(A) Representative calcium imaging traces of at least ten individual sensory neurons are shown for each line at 4 weeks of differentiation. KCl indicates the time at which the depolarizing stimulus was added to the cultures.

(B and C) Sensory neurons from each of the LRRK2 G2019S iPSC lines displayed a significantly reduced response to KCl-induced depolarization at 4 and 6 weeks of differentiation compared to controls and SNCA (3×). ^∗p < 0.01 by ANOVA to the controls and Student’s t test to SNCA (3×); n = 6 independent experiments, totaling ≥100 neurons from each iPSC line.

See also Figure S4.