Extended Data Figure 3. Representative electrophysiological recordings from wild-type and I159C R284C TRAAK.

In these experiments and those in Fig. 2, inside-out patches from cells expressing wild-type or mutant TRAAK channels were excised and perfused with reducing bath solution (with 10 mM DTT). After stabilization of the patch (TRAAK channels exhibit a gradual run-up of current following excision to an equilibrium value, e.g. Extended Data Fig. 4), the perfusion solution was switched to oxidizing bath solution (no DTT). (a,b) Representative voltage family from a I159C R284C TRAAK patch during perfusion of (a) reducing and (b) oxidizing solution. The voltage family protocol is illustrated. (c,d) Same as (a,b) but from a wild-type TRAAK patch. (e,f) Current response (upper) to pressure application (lower) at 0 mV from the same I159C R284C TRAAK patch during perfusion of (e) reducing or (f) oxidizing bath solution. (g,h) Same as (e,f), but from a wild-type TRAAK patch. Scale is shown between each pair of recordings in reducing and oxidizing bath solutions.