Table 1.
Sample | Raw reads | Trimmed reads | Confident reads | Total genus | Confident genus | No. of contigs |
lcl/mrl. ≥1.5 |
lcl/mrl ≥2 |
lc identity ≥97% |
---|---|---|---|---|---|---|---|---|---|
S1 | 31,035 | 29,170 | 27,423 | 8 | 4 | 4 | 4 | 3 | 4 |
S2 | 38,100 | 35,007 | 30,242 | 144 | 28 | 23 | 15 | 6 | 11 |
S3 | 39,881 | 35,456 | 10,909 | 191 | 62 | 53 | 28 | 12 | 27 |
S4 | 91,44 | 4,590 | 478 | 65 | 9 | 9 | 4 | 3 | 3 |
S5 | 25,314 | 16,857 | 3,059 | 218 | 38 | 34 | 24 | 12 | 14 |
S6 | 14,709 | 8,347 | 854 | 117 | 18 | 13 | 5 | 2 | 2 |
S7 | 13,810 | 8,262 | 983 | 128 | 20 | 17 | 4 | 3 | 4 |
S8 | 11,379 | 9,214 | 2,145 | 230 | 47 | 37 | 18 | 5 | 18 |
S9 | 9,924 | 8,277 | 1,374 | 193 | 33 | 30 | 13 | 5 | 16 |
S10 | 12,084 | 8,216 | 1,685 | 124 | 23 | 20 | 11 | 4 | 7 |
A confident read is one with a ≥80 classification score. A genus is counted when there is a confident read. A confident genus is one with ≥10 confident reads. Confident reads of each confident genus were assembled and the longest contig (lc) analyzed. The last column shows the number of longest contigs with a ≥97% alignment identity to the 16S rDNA references. Abbreviations: longest contig length (lcl), mean read length (mrl).