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In-silico sensitivity of the six primers.
| Primer | No. of 16S rDNA sequences covering the primer position | No. of amplifiable sequences | Percentage |
|---|---|---|---|
| A | 468049 | 411649 | 87.9% |
| B | 2140848 | 1873932 | 87.5% |
| C | 1877993 | 1796209 | 95.6% |
| D | 1787993 | 1424757 | 79.7% |
| E | 1003626 | 915216 | 91.2% |
| F | 61634 | 47650 | 77.3% |
| At least one | 2472276 | 2455930 | 99.3% |
