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. 2015 Oct 14;67(1):239–257. doi: 10.1093/jxb/erv454

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Interaction between PpCBF and PpDAM promoter as determined by Y1H assay. (A) Upstream regions of PpDAM A- and C-type promoters showing location of C-repeat/DRE transcription factor binding site. (B) Y1H assays showing interaction between PpCBF and PpDAM promoters. (C) The promoter of PpDAM with mutated C-repeat/DRE site was synthesized artificially and was inserted into pAbAi plasmid for Y1H assays. The pAbAi vector ligated to the promoter of PpDAM with non-mutated C-repeat/DRE site as a positive control. Y1H assays showed interaction between PpCBF and promoters of PpDAM with mutated C-repeat/DRE site and non-mutated C-repeat/DRE site. (This figure is available in colour at JXB online.)