The genotypes of the Spark X Rialto 1DL NILs using markers TaBradi2g14790 (A), TaBradi2g14340 (B), TaBradi2g14290 (C), and TaBradi2g14210 (D). The letters A and B, which are followed by numbers, indicate the NILs carrying the Spark and Rialto allele, respectively. Spa and Ria are Spark and Rialto wheat cultivars, respectively. The solid horizontal line from A1 to B5 designates the first NIL pair, the dotted horizontal line (A6–B9) designates the second NIL pair, the solid horizontal line flanked by diamond shapes (A10–B14) is the third NIL pair and the dashed horizontal line flanked by circles (A15–B18) is the fourth NIL pair. The letters E and L at the bottom of the Figure indicate early flowering (E) and late flowering (L), respectively, of the NILs. The fragment size markers are shown in kilobases (kb). We showed the flowering time differences both in the field and controlled environments for these NILs in our earlier report (Zikhali et al., 2014).