Fig. 7.

Cotyledon movement in WT, COPT1 ^OE, and spl7 seedlings. WT and COPT1 ^OE seedlings were grown for 5 d in (A) Cu deficiency (1/2 MS; –Cu) and (B) Cu excess (1/2 MS with 10 µM CuSO₄; +Cu) under a neutral photoperiod. The seedlings were transferred to new plates (1/2 MS containing 1.5% phytoagar). After 24h, photographs were taken under continuous light for 6 d, every 2h. Data shown have been corrected for position changes due to plant growth and cotyledon senescence by subtracting these trends, calculated according to linear regression of the daily minimum values. Mean values ±SD of n=5 are shown. The subjective night is shown as grey areas. Mean periods and amplitudes of WT plants were 24.4±0.3 and 9.1±1.3 in –Cu and 24.2±0.4 and 8.3±1.4 in +Cu. (C) Period and (D) amplitude of the cotyledon movement in the spl7 mutant. WT, spl7-1, spl7-2, and CSPL7 (spl7-2 line complemented with SPL7) seedlings were grown for 5 d in Cu sufficiency (1/2 MS with 0.5 µM Cu; Ctrl, white bars) and Cu deficiency (1/2 MS; –Cu, grey bars) and processed as in (A and B) but photographs were taken every 30 min. a.u., arbitrary units. Means with a different letter are significantly different (P<0.05).