Fig. 1.

Effect of exogenous GR24 on LR development near the root–shoot junction.

(A) Distance to the first emerged LR in Col-0 (top) and max2-1 (bottom). (B) Total number of prebranch sites under mock (white bars) and GR24 treatment (gray bars), 4 and 9 DAG in Col-0 (top) and max2-1 (bottom). (C) Percentage of initiated patches under mock and 1 μM GR24 treatments in Col-0 (top) and max2-1 (bottom) at 9 DAG. (D) Percentage of emerged patches under mock and GR24 treatment in Col-0 (top) and max2-1 (bottom). (A–D) Data presented are means ± standard error (SE) of three biological repeats (n>20). *P<0.001, according to the Student’s t-test. (E, F) Stages of LR primordia via GATA23:GUS staining in Col-0 under mock (left) and GR24 treatment (right) at 4 DAG (E) and 9 DAG (F). All events, possibly leading to emerged LRs, were scored in individual plants, color-coded, and for each plant, vertically ordered from the closest to the hypocotyl (up) downward to the meristem (down). The root fragments used for analysis were comparable in length. Data of one representative experiment are shown. The experiments were repeated three times with similar results.