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. 2015 Oct 31;67(1):379–389. doi: 10.1093/jxb/erv478

Fig. 3.

Fig. 3.

Interrelation between the polar auxin transport and the GR24 effect on LR development. (A–C) LRD of pin7-1, pin1-613, pin3-3, and pin5-3 mutants compared with WT grown in the presence or absence of GR24. Data presented are means ± SE of three biological repeats (n>20). (D) Relative PIN1 expression in 5-d-old seedlings under mock and GR24 treatment as determined by qRT-PCR. Material was harvested separately from the upper part (old, above the first emerged LR) and the lower part (young) of the root. ***P<0.001, *P<0.05, according to ANOVA mixed-model statistical analyses.(E) pPIN1:GUS expression patterns of plants grown with and without GR24, 7 d after growth. Frames until the first emerged LR are shown. (F) Expression of PIN1 with pPIN1:GUS plants during different stages of LR development under mock and GR24 treatment. The panels indicated by the asterisk display the first emerged LR and those above the asterisk correspond to the LR primordia near the root–shoot junction. Scale bars=40 µm.