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. 2015 May 4;20(6):521–542. doi: 10.1111/gtc.12246

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© 2015 The Authors. Genes to Cells published by Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Reduction in mRNA and GAG levels by silencing of CG15110 (Dext3) and CG32300 (dXylT). (A, B) The mRNA levels of CG15110 and CG32300 in Act5C-GAL4/UAS-IR-CG15110 (A), Act5C-GAL4/UAS-IR-CG17772 (B), and Actin5C-GAL4/+ (as control in A and B) were quantified by real-time PCR. (C–E) Typical chromatograms of GAG-derived oligosaccharides in the third instar larvae of Actin5C-GAL4/+ (C), Act5C-GAL4/UAS-IR-CG15110 (D), and Act5C-GAL4/UAS-IR-CG17772 (E). HS, chromatograms of unsaturated disaccharides from heparan sulfate. CS, chromatograms of unsaturated disaccharides from low-sulfated chondroitin 4-sulfate. Peaks: 1, ΔUA-GlcNAc; 2, ΔUA-GlcNS; 3, ΔUA-GlcNAc6S; 4, ΔUA-GlcNS6S; 5, ΔUA2S-GlcNS; 6, ΔUA2S-GlcNS6S; 7, ΔDi-0S; and 8, ΔDi-4S. The peak heights in each chromatogram reflect the amount of oligosaccharides and can be compared between different genotypes.