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. 2015 May 4;20(6):521–542. doi: 10.1111/gtc.12246

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© 2015 The Authors. Genes to Cells published by Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Identification of CG33145 as a novel β1,3galactosyltransferase of N-glycosylation. (A) The structures of an acceptor substrate E2 and its Gal extended forms, E4 and E5, which have one and two terminal Gal moieties, respectively. (B) FLAG-CG33145-PB and FLAG-BAP expressed in insect cells were purified and detected by anti-FLAG antibody. (C) Products of the CG33145-mediated reaction were analyzed by HPLC. CG33145 produced E4 and E5. (D) β1,3galactosidase treatment of reaction product E5. The E5 product peak shifted to peaks corresponding to E4 and E2 after β1,3galactosidase treatment.