Table 2.
Off-target probability score (OTPS) and phenotypes caused by whole-body gene silencing
| Family of proteins/protein name | Protein/gene name | CG No. | OTPS | Act5C |
|---|---|---|---|---|
| Glycosyltransferase | ||||
| N-acetylgalactosaminyltransferase | ||||
| UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase | pgant1/GalNAc-T1 | CG8182 | 1 | Lethal |
| pgant2 | CG3254 | 0 | Lethal | |
| pgant3 | CG4445 | 2 | Viable | |
| pgant4 | CG31956 | 0 | Lethal | |
| pgant5 | CG31651 | 0 | Lethal | |
| pgant6 | CG2103 | 0 | Lethal | |
| pgant7/GalNAc-T2 | CG6394 | 1 | Lethal | |
| pgant8 | CG7297 | 0 | Lethal | |
| pgant35A | CG7480 | 0 | Lethal | |
| dppGalNAcT9 | CG30463 | 2 | Lethal | |
| dppGalNAcT10 | CG10000 | 1 | N.T. | |
| CG31776 | no line | N.T. | ||
| dppGalNAcT11 | CG7304 | no line | N.T. | |
| dppGalNAcT12 | CG7579 | 1 | N.T. | |
| α1,4-N-acetylgalactosaminyltransferase | α4GT1 | CG17223 | 0 | Viable |
| α4GT2 | CG5878 | 0 | N.T. | |
| β1,4-N-acetylgalactosaminyltransferase | β4GalNAcTA | CG8536 | 0 | N.T. |
| β4GalNAcTB | CG14517 | 1 | Viable | |
| N-acetylglucosaminyltransferase | ||||
| UDP-GlcNAc:polypeptide O-β-N-acetylglucosaminyltransferase | dO-GnT/Sxc | CG10392 | 0 | Lethal |
| α3-D-mannoside-β1,2-N-acetylglucosaminyltransferase | dMGAT1/Mgat1 | CG13431 | 2 | Lethal |
| α6-D-mannoside-β1,2-N-acetylglucosaminyltransferase | dMGAT2/Mgat2 | CG7921 | 1 | Lethal |
| β4-D-mannoside-β1,4-N-acetylglucosaminyltransferase | dMGAT3 | CG31849 | 1 | Lethal |
| α3-D-mannoside-β1,4-N-acetylglucosaminyltransferase | dMGAT4-1 | CG9384 | 2 | Viable |
| dMGAT4-2 | CG17173 | 0 | Lethal | |
| i-β1,3-N-acetylglucosaminyltransferase | diβ3GnT1 | CG3253 | 1 | Viable |
| diβ3GnT2 | CG9171 | 38 | N.T. | |
| diβ3GnT3 | CG15483 | 0 | Viable | |
| diβ3GnT4 | CG11149 | 0 | Lethal | |
| diβ3GnT5 | CG9996 | 0 | Lethal | |
| diβ3GnT6 | CG11388 | 0 | Lethal | |
| β1,3-N-acetylglucosaminyltransferase | Brn | CG4934 | 0 | Lethal |
| Fng | CG10580 | 0 | Lethal | |
| β1,3-N-acetylglucosaminyltransferase or | dβ3GnT or GalT1 | CG33145 | 0 | Lethal |
| β1,3-galactosyltransferase | dβ3GnT or GalT2 | CG11357 | 2 | Lethal |
| dβ3GnT or GalT3 | CG3038 | 0 | Viable | |
| dβ3GnT or GalT4 | CG8668 | 0 | Lethal | |
| dβ3GnT or GalT5 | CG8673 | 11 | N.T. | |
| Dolichyl phosphate N-acetylglucosaminyltransferase | dAlg14 | CG6308 | no line | N.t. |
| dAlg7 | CG5287 | 0 | Lethal | |
| dAlg13 | CG14512 | 0 | Lethal | |
| Chondroitin synthase | ||||
| Chondroitin synthase | dCHSY | CG9220 | 2 | n.t. |
| Chondroitin polymerization factor | dCHPF | CG43313 | 0 | Lethal |
| Chondroitin N-acetylgalactosaminyltransferase | dCSGalNAcT1 | CG12913 | 2 | Viable |
| Chitin synthase | Chitin Syn1/Kkv | CG2666 | 0 | Lethal |
| Chitin Syn2 | CG7464 | 0 | Lethal | |
| Fucosyltransferase | ||||
| α1,3/1,4-fucosyltransferase or | FucTA | CG6869 | 6 | N.T. |
| α1,3-fucosyltransferase | FucTB | CG4435 | 0 | Lethal |
| FucTD | CG9169 | 1 | Lethal | |
| FucTC | CG40305 | no line | N.T. | |
| α1,6-fucosyltransferase | dα6Fut/FucT6 | CG2448 | 1 | Lethal |
| Protein O-fucosyltransferase | OFut1 | CG12366 | 0 | Lethal |
| OFut2 | CG14789 | 0 | Viable | |
| Galactosyltransferase | ||||
| GAGβ1,4-galactosyltransferase I | dGAGβ4GalTI/β4GalT7 | CG11780 | 0 | Lethal |
| GAGβ1,3-galactosyltransferase II | dGAGβ3GalTII | CG8734 | 1 | Lethal |
| core1β1,3-galactosyltransferase | dC1GalT1/C1GalTA | CG9520 | 0 | Viable |
| dC1GalT2 | CG8708 | 1 | Lethal | |
| dC1GalT3 | CG18558 | 0 | N.T. | |
| dC1GalT4 | CG2975 | 8 | N.T. | |
| dC1GalT5/Tgy | CG7440 | 0 | Lethal | |
| dC1GalT6 | CG34056 | 8 | N.T. | |
| CG34057 | 8 | N.T. | ||
| dC1GalT7 | CG3119 | 2 | N.T. | |
| dC1GalT8 | CG2983 | 2 | Viable | |
| dC1GalT9 | CG9109 | 1 | Lethal | |
| Glucosyltransferase | ||||
| Dolichyl phosphate glucosyltransferase | dAlg5/Wol | CG7870 | 2 | N.T. |
| Dolichyl pyrophosphate glucosyltransferase | dAlg6/Gny | CG5091 | 0 | N.T. |
| dAlg8 | CG4542 | 2 | N.T. | |
| dAlg10 | CG32076 | 1 | N.T. | |
| Glucosylceramide synthase | dGlcCerT/GlcT-1 | CG6437 | 1 | Lethal |
| Protein O-glucosyltransferase | Rumi | CG31152 | no line | N.T. |
| Ugt | CG6850 | no line | N.T. | |
| Glucuronyltransferase | ||||
| GAG glucuronyltransferase I | dGlcAT-I | CG32775 | 0 | Lethal |
| β1,3-glucuronyltransferase | dGlcAT-BSI/GlcAT-S | CG3881 | 0 | Viable |
| dGlcAT-BSII/GlcAT-P | CG6207 | 24 | N.T. | |
| CG30438 | 0 | Viable | ||
| Hereditary multiple exostoses (EXT) protein | dExt1/Ttv | CG10117 | 0 | Lethal |
| dExt2/Sotv | CG8433 | 0 | Lethal | |
| dExt3/Botv | CG15110 | ? | Lethal | |
| Mannosyltransferase | ||||
| β1,4-mannosyltransferase | β1,4ManT/Egh | CG9659 | 0 | N.T. |
| Dolichyl pyrophosphate mannosyltransferase | dAlg1 | CG18012 | 0 | Lethal |
| dAlg2 | CG1291 | 2 | N.T. | |
| dAlg11 | CG11306 | 0 | N.T. | |
| dAlg3/l(2)not | CG4084 | 0 | N.T. | |
| dAlg9 | CG11851 | no line | N.T. | |
| dAlg12 | CG8412 | 0 | N.T. | |
| dDPM | CG10166 | 0 | N.T. | |
| Protein O-mannosyltransferase | dPomt1/Rt | CG6097 | 0 | Lethal |
| dPomt2/Tw | CG12311 | 0 | Lethal | |
| Sialyltransferase | ||||
| Galactoside α2,6-sialyltransferase | dST6Gal I | CG4871 | 3 | N.T. |
| Xylosyltransferase | ||||
| Peptide-O-xylosyltransferase | dXylT/Oxt | CG32300 | 0 | Lethal |
| Oligosaccharyltransferase | ||||
| Oligosaccharyltransferase | OST | CG33303 | 0 | N.T |
| CG9022 | no line | N.T | ||
| CG7830 | 0 | N.T | ||
| CG6370 | no line | N.T | ||
| CG13393 | no line | N.T | ||
| STT | CG1518 | 0 | N.T | |
| STT | CG7748 | no line | N.T | |
| Fukutin-related protein | CG15651 | 0 | Lethal | |
| Sulfotransferase | ||||
| Chondroitin 4-O-sulfotransferase | dC4ST | CG31743 | 0 | Viable |
| N-acetylgalactosamine-4-O-sulfotransferase | d4ST1 | CG14024 | 6 | N.T. |
| d4ST2 | CG13937 | 0 | Viable | |
| N-acetylgalactosamine/N-acetylglucosamine/galactose | d6ST1 | CG31637 | 0 | N.T. |
| 6-O-sulfotransferase | d6ST2 | CG9550 | 0 | Lethal |
| Heparan sufate sulfotransferase | Pipe | CG9614 | 1 | Lethal |
| Heparan N-deacetylase/N-sulfotransferase | Sfl | CG8339 | 1 | N.T. |
| Heparan sulfate 2-O-sulfotransferase | HS2ST | CG10234 | 0 | Viable |
| Heparan sulfate 6-O-sulfotransferase | dHS6ST | CG4451 | 0 | N.T. |
| Heparan sulfate d-glucosaminyl 3-O-sulfotransferase | dHS3OSTA | CG33147 | 2 | N.T. |
| dHS3OSTB | CG7890 | 3 | N.T. | |
| C5 epimerase | Heparan sulfate C5-epimerase | CG3194 | 0 | lethal |
| Sugar-nucleotide transporter | ||||
| GDP-Fuc transporter (Golgi) | Gfr/Nac | CG9620 | 0 | N.T. |
| GDP-Fuc/UDP-GlcNAc/UDP-Xyl transporter (ER) | Efr | CG3774 | no line | N.T. |
| UDP-Gal/UDP-GalNAc transporter | Csat | CG2675 | 0 | Lethal |
| UDP-sugar transporter | Frc | CG3874 | 2 | Lethal |
| Sugar-nucleotide transporter | Meigo | CG5802 | 0 | Lethal |
| PAPS transporter | Sll | CG7623 | 0 | Lethal |
| dPAPST2 | CG7853 | 2 | Lethal | |
| Core protein | ||||
| Glypican | Dally | CG4974 | 4 | N.T. |
| Glypican | Dlp | CG32146 | 2 | Lethal |
| Dystroglycan | αDystroglycan | CG18250 | 1 | N.T. |
| Syndecan | dSdc | CG10497 | 3 | N.T. |
| Perlecan | dPerlecan/Trol | CG33950 | 0 | N.T. |
| Glycosidase | ||||
| α-mannosidase I | α-Man-I | CG42275 | no line | N.T. |
| α-Man-II | CG18802 | 0 | Lethal | |
| β-N-acetylglucosaminidase | Fdl | CG8824 | no line | N.T. |
no line: no UAS-IR line; N.T.: not tested.